A simple, rapid and precise UV spectrophotometric method for simultaneous estimation of cefuroxime axetil and probenecid from combined solid dosage form has been developed. Cefuroxime axetil and probenecid interfere with the UV absorption of each other. However, as they show different absorption maxima, use of modified Beer’s law for the simultaneous estimation of both the drugs was explored. As both the drugs have different pharmacopoeial in-vitro dissolution media, simultaneous equations were developed in both the systems. The accuracy and precision of the method was determined and validated statistically. The method showed good reproducibility and recovery with percent relative standard deviation less than 2%. This method was successfully applied for determining the assay and in vitro dissolution of a marketed formulation.